Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.0K
Cross-Sectional Research01:50

Cross-Sectional Research

12.4K
In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
12.4K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

12.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
12.8K
Method of Sections01:30

Method of Sections

1.2K
Consider a truss structure, as shown in the figure.
1.2K
Fixation and Sectioning01:03

Fixation and Sectioning

7.5K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
7.5K
Surface Tension and Surface Energy01:16

Surface Tension and Surface Energy

3.2K
When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gut microbiota dysbiosis in endometriosis: mechanistic insights and gut microbiota-targeted therapeutic strategies.

Frontiers in microbiology·2026
Same author

Augmenting large language models with clinical knowledge graph for personalized perioperative fluid therapy question answering.

PLOS digital health·2026
Same author

Aberration-balanced initial structure design of extreme ultraviolet lithography objective systems via hybrid optimization.

Applied optics·2026
Same author

Nitazoxanide cooperates with cytarabine to inhibit cytarabine-resistant acute myeloid leukemia progression via mitochondrial dysfunction and PLK1 suppression.

Biochemical pharmacology·2026
Same author

PEPRKD-depression: A knowledge database supporting evidence-based personalized exercise prescription recommendations in depression.

Digital health·2026
Same author

Robust Thermochromic Photothermal Coating with Ultraslippery Anti-icing/Deicing and All-Season Temperature Regulation Performance.

Research (Washington, D.C.)·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K

Accurate surface profilometry using differential optical sectioning microscopy with structured illumination.

Zhongye Xie, Yan Tang, Jinhua Feng

    Optics Express
    |May 5, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Differential optical sectioning microscopy with structured-illumination (DOSM-SI) enhances 3D measurement precision. This technique uses two detectors to create a differential depth response curve, improving resolution and accuracy for sample surface reconstruction.

    More Related Videos

    Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
    14:11

    Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

    Published on: May 4, 2014

    16.4K
    Phase Contrast and Differential Interference Contrast DIC Microscopy
    06:49

    Phase Contrast and Differential Interference Contrast DIC Microscopy

    Published on: August 6, 2008

    54.0K

    Related Experiment Videos

    Last Updated: Jan 25, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.1K
    Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
    14:11

    Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

    Published on: May 4, 2014

    16.4K
    Phase Contrast and Differential Interference Contrast DIC Microscopy
    06:49

    Phase Contrast and Differential Interference Contrast DIC Microscopy

    Published on: August 6, 2008

    54.0K

    Area of Science:

    • Optical microscopy
    • 3D metrology
    • Structured illumination techniques

    Background:

    • Traditional optical sectioning microscopy methods face limitations in axial precision.
    • The contrast depth response curve (CDR) is sensitive to height variations, impacting measurement accuracy.

    Purpose of the Study:

    • To introduce and validate a novel differential optical sectioning microscopy with structured-illumination (DOSM-SI) method.
    • To enhance axial precision and resolution for three-dimensional (3D) measurements.
    • To improve the accuracy of sample surface reconstruction.

    Main Methods:

    • Implementation of DOSM-SI utilizing two charge-coupled detectors (CCDs) at different axial positions.
    • Generation of a differential depth response curve (DCDR) by subtracting signals from the two CCDs.
    • Accurate extraction of the zero-crossing point of the DCDR using line-fitting techniques.

    Main Results:

    • The DCDR exhibits a steeper slope around the zero-crossing point compared to conventional methods.
    • DOSM-SI achieves enhanced resolution and precision in 3D surface reconstruction.
    • Experimental and theoretical analyses confirm the feasibility and effectiveness of the proposed method.

    Conclusions:

    • DOSM-SI offers a significant improvement in axial precision for 3D measurements.
    • The novel DCDR approach effectively overcomes limitations of traditional CDR methods.
    • This technique provides a robust platform for high-resolution and high-precision optical metrology.