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TASBE Flow Analytics: A Package for Calibrated Flow Cytometry Analysis.

Jacob Beal1, Cassandra Overney1,2, Aaron Adler1

  • 1Raytheon BBN Technologies , Cambridge , Massachusetts 02138 , United States.

ACS Synthetic Biology
|May 5, 2019
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Summary
This summary is machine-generated.

The TASBE Flow Analytics package simplifies complex flow cytometry data analysis for synthetic biology. This free, open-source tool offers high-throughput processing, calibration, and reporting for large datasets.

Keywords:
batch analysisflow cytometrysoftware toolsunit calibrationuser interface

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Area of Science:

  • Biotechnology
  • Computational Biology
  • Cellular Analysis

Background:

  • Flow cytometry is crucial for high-throughput cell measurement.
  • Analyzing synthetic biology data requires complex, multistage workflows.
  • Existing methods can be inaccessible or difficult to implement.

Purpose of the Study:

  • To provide an accessible, open-source software package for flow cytometry data analysis in synthetic biology.
  • To streamline the quantification and comparison of synthetic biology devices and circuits.
  • To facilitate high-throughput analysis of large experimental datasets.

Main Methods:

  • Development of the TASBE Flow Analytics package.
  • Implementation of calibration, filtering, and statistical analysis workflows.
  • Integration with user-friendly interfaces (Excel, Matlab, Python) and automated workflows.

Main Results:

  • The package processes experimental samples and controls to yield calibrated data.
  • It generates quantitative analyses, comparisons, and automated figures.
  • Detailed human-readable and machine-readable diagnostic reports are produced.

Conclusions:

  • TASBE Flow Analytics offers a powerful, free, and accessible solution for synthetic biology flow cytometry data.
  • The package enhances the effective use of flow cytometry for device and circuit quantification.
  • It supports diverse implementation needs, from interactive sessions to automated pipelines.