Related Experiment Video
Updated: Jan 25, 2026
13:10
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
Published on: August 1, 2014
12.3K
Graft Immunocomplex Capture Fluorescence Analysis Can Detect Intragraft Anti-Major Histocompatibility Complex
T Nakamura1, T Shirouzu2, S Kawai2
1Department of Organ Transplantation and General Surgery, Kyoto Prefectural University of Medicine, Kajii-cho 465, Kamigyo-ku, Kyoto-prefecture, Japan.
Transplantation Proceedings
|May 5, 2019
Summary
Immunocomplex capture fluorescence analysis successfully detected donor-specific antibodies (DSA) in mouse cardiac transplants. This method, adapted for murine models, aids in understanding antibody-mediated rejection mechanisms.
Area of Science:
- Transplantation immunology
- Immunodiagnostics
Background:
- Immunocomplex capture fluorescence analysis is used for detecting intragraft donor-specific antibodies (DSA) in human transplants.
- Intragraft DSA is a key area of research, but its assessment in murine organ transplantation is not reported.
Purpose of the Study:
- To assess the applicability of immunocomplex capture fluorescence analysis for detecting intragraft DSA in a murine cardiac transplantation model.
- To establish a method for studying DSA in mice to advance understanding of antibody-mediated rejection.
Main Methods:
- A presensitized mouse cardiac transplantation model was utilized.
- Anti-MHC class I/II antibodies immobilized on Luminex beads captured MHC/DSA complexes.
- Detection involved phycoerythrin-conjugated antimouse IgG antibodies.
Main Results:
- Luminex beads detected class I DSA in cardiac allografts.
- MHC class I was abundant in allografts, while MHC class II expression was minor.
- MHC/class II DSA complexes were detected in recipient splenocytes and serum.
Conclusions:
- Graft immunocomplex capture fluorescence analysis is applicable to murine cardiac transplantation.
- This murine model facilitates mechanistic studies of DSA and antibody-mediated rejection.
Related Concept Videos
01:21Mass Spectrometry: Complex Analysis
1.7K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.7K
01:19Major Organs of the Digestive System
9.1K
The digestive system is responsible for the ingestion of food, secretion of enzymes, mixing and digesting food, absorption of the nutrients and defecation. The human digestive system consists of two major parts: the gastrointestinal tract and the accessory digestive organs.
Gastrointestinal tract:
Gastrointestinal tract:
9.1K
01:28Major Losses in Pipes
1.9K
When a fluid flows through a pipe, it experiences energy losses due to frictional resistance along the pipe walls, known as major losses. These energy losses result in a pressure drop, which varies based on the flow conditions — whether laminar or turbulent — and the specific physical properties of the fluid and pipe.
Fluid flow can be classified as laminar or turbulent, primarily based on the Reynolds number. This dimensionless number reflects the relative influence of inertial to viscous...
Fluid flow can be classified as laminar or turbulent, primarily based on the Reynolds number. This dimensionless number reflects the relative influence of inertial to viscous...
1.9K
01:27Major Hormones and Their Functions
1.8K
Hormones, the biochemical messengers produced by endocrine glands, are pivotal in regulating bodily functions and maintaining homeostasis. Each hormone's balance is crucial; imbalances can lead to significant physiological disruptions. Major hormones include oxytocin, cortisol, epinephrine, estrogen, testosterone, thyroxine, growth hormone, insulin, and glucagon.
Oxytocin, produced in the hypothalamus and released by the pituitary gland, plays a role in social bonding, childbirth, and...
Oxytocin, produced in the hypothalamus and released by the pituitary gland, plays a role in social bonding, childbirth, and...
1.8K
01:20Anatomy of the Brain: Major Regions
10.0K
The brain is the most complex organ in the human body. It consists of four main parts: the cerebrum, diencephalon, cerebellum, and brainstem.
The cerebrum is the largest section of the brain and divides into left and right hemispheres, separated by a deep fissure. The cerebral outer layer of grey matter — the cerebral cortex — comprises elevations called gyri and shallow groves called sulci. The inner portion of white matter includes long nerve fibers known as axons, which connect...
The cerebrum is the largest section of the brain and divides into left and right hemispheres, separated by a deep fissure. The cerebral outer layer of grey matter — the cerebral cortex — comprises elevations called gyri and shallow groves called sulci. The inner portion of white matter includes long nerve fibers known as axons, which connect...
10.0K
01:28Major Somatic Sensory Pathways
2.5K
Sensory impulses related to touch, pressure, vibration, and proprioception from various body parts, such as the limbs, trunk, neck, and posterior head, travel to the cerebral cortex through the posterior column-medial lemniscus pathway. The pathway’s name derives from the two white-matter tracts that convey the impulses: the spinal cord's posterior column and the brainstem's medial lemniscus. First-order sensory neurons extend their axons into the spinal cord, forming the...
2.5K