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Molecular characterisation of methicillin-resistant Staphylococcus pseudintermedius from dogs and the description of

Martina Krapf1, Elke Müller2, Annett Reissig2

  • 1LABOKLIN GmbH&Co.KG, Bad Kissingen, Germany.

Veterinary Microbiology
|May 5, 2019
PubMed

Insights

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is increasingly found in dogs. This study characterized MRSP isolates in Germany, finding the ST71-SCCmec III strain to be predominant, suggesting a single dominant strain causes most infections.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Molecular Epidemiology

Background:

  • Increasing prevalence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in canine populations.
  • Need for characterization of MRSP isolates to understand resistance gene and SCCmec element distribution.

Purpose of the Study:

  • Characterize MRSP isolates from clinical diagnostics in a German laboratory.
  • Assess the abundance of specific resistance genes and SCCmec elements in these isolates.
  • Identify the predominant MRSP strain circulating in Germany.

Main Methods:

  • Analysis of 97 MRSP isolates from 96 dogs using microarrays.
  • Detection of resistance genes including mecA, blaZ, aacA-aphD, aphA3, sat, erm(B), tet(K), tet(M), and cat.
  • Identification of SCCmec elements (types III, IV, V) and pseudo-SCCmec elements.

Main Results:

  • All isolates harbored mecA and blaZ.
  • High prevalence of other resistance markers: aacA-aphD, aphA3, sat, erm(B) (>80%); tet(K), tet(M), cat (>20%).
  • SCCmec III elements were most common (59 isolates), followed by SCCmec IV (21) and V (15).

Conclusions:

  • The study identified a high degree of uniformity among MRSP isolates, suggesting a single dominant strain.
  • The predominant strain was identified as ST71-SCCmec III, consistent with strains prevalent in Western Europe.
  • This finding highlights the importance of molecular surveillance for tracking MRSP epidemiology in dogs.
Keywords:
MRSPMethicillin resistanceMicroarrayMolecular characterisationSCCmecStaphylococcus pseudintermediuss

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