Related Experiment Video
Updated: Jan 25, 2026

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
Distinct immunocyte-promoting and adipocyte-generating stromal components coordinate adipose tissue immune and
Raul German Spallanzani1, David Zemmour1, Tianli Xiao1
1Department of Immunology, Harvard Medical School and Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115, USA.
Abstract:
Regulatory T cells (Tregs) are key brakes on the visceral adipose tissue (VAT) inflammation that regulates local and systemic metabolic tenor. Breakdown of this regulation promotes type 2 diabetes. The cytokine IL-33 expands and sustains the unique Treg population residing within VAT. Here, relying on single-cell RNA sequencing, we identified the major IL-33 producers in VAT to be particular mesenchymal stromal cell subtypes, related to but distinct from adipocyte progenitor cells. We explored modulation of the VAT stromal cell landscape with physiologic variables such as age and sex, as well as its remodeling in pathogenic states like obesity. Last, we uncovered a VAT Treg:stromal cell negative regulatory loop that keeps the potent effect of IL-33 under rein.
Related Concept Videos
Vector Components in the Cartesian Coordinate System
Coordination Number and Geometry
The Eukaryotic Promoter Region
Coordination Compounds and Nomenclature
Lattice Centering and Coordination Number
Types of Unit Cells
Imagine taking a large number of identical...
Equations of Motion: Rectangular Coordinates and Cylindrical Coordinates
When a particle moves relative to an inertial frame, the equations of motion can be expressed using rectangular components. If the motion is confined to the x-y plane, the equations having the x and y coordinates only can be used to simplify the mathematical representation.
However, when particles...

