Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simple method for screening aflatoxin-producing molds by UV photography.

K Yabe, Y Ando, M Ito

    Applied and Environmental Microbiology
    |February 1, 1987
    PubMed
    Summary

    UV photography can identify aflatoxin-producing molds by their colony color and UV absorption. This simple method rapidly screens for aflatoxins B1 and G1 in fungal cultures.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Comparison of renal biomarkers with glomerular filtration rate in susceptibility to the detection of gentamicin-induced acute kidney injury in dogs.

    Journal of comparative pathology·2014
    Same author

    Silent period and H reflex from soleus muscle as an index in a neuro-muscular function after reconstruction of anterior cruciate ligament.

    Electromyography and clinical neurophysiology·2009
    Same author

    Isolation of microorganisms and substances inhibitory to aflatoxin production.

    Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2008
    Same author

    Neuromuscular function after reconstruction of anterior cruciate ligament--a case study using evoked electromyography.

    Electromyography and clinical neurophysiology·2008
    Same author

    Implications for using H-max/M-max ratio in H-reflex parameters for elderly subjects compared with young subjects.

    Electromyography and clinical neurophysiology·2006
    Same author

    Purification and Characterization of O-Methyltransferase I Involved in Conversion of Demethylsterigmatocystin to Sterigmatocystin and of Dihydrodemethylsterigmatocystin to Dihydrosterigmatocystin during Aflatoxin Biosynthesis.

    Applied and environmental microbiology·2005

    Area of Science:

    • Mycology
    • Food Safety
    • Analytical Chemistry

    Background:

    • Aflatoxins are toxic secondary metabolites produced by certain fungi.
    • Accurate and rapid detection of aflatoxin-producing molds is crucial for food safety.
    • Current detection methods can be time-consuming or require specialized equipment.

    Purpose of the Study:

    • To develop a simple, safe, and rapid method for screening aflatoxin-producing molds.
    • To utilize UV absorption properties of aflatoxins for visual identification.
    • To correlate UV absorption patterns with aflatoxin production.

    Main Methods:

    • Monitoring UV absorption of fungal colonies grown on GY agar medium using UV photography.
    • Identifying aflatoxin-producing molds by characteristic colony coloration (gray/black) versus non-producing molds (white).
    • Confirming the identity of UV-absorbing compounds as aflatoxins B1 and G1 via cellophane transplantation and thin-layer chromatography.

    Main Results:

    • Aflatoxin-producing molds exhibited distinct UV absorption patterns in UV photographs.
    • UV-absorbing compounds were identified primarily as aflatoxins B1 and G1 excreted into the agar.
    • UV absorption was dependent on the carbon source and inhibited by specific chemical inhibitors, confirming its link to aflatoxin biosynthesis.

    Conclusions:

    • UV photography provides a straightforward and effective method for preliminary screening of aflatoxin-producing molds.
    • The technique offers a rapid, visual alternative to conventional detection methods.
    • This approach can significantly aid in early identification and management of aflatoxin contamination risks.

    Related Experiment Videos