Related Experiment Video

Updated: Jan 25, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
09:29

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

18.8K

Triple-hit therapeutic approach for triple negative breast cancers using docetaxel nanoparticles, EN1-iPeps and RGD

Anabel Sorolla1, Edina Wang1, Tristan D Clemons2

  • 1Cancer Epigenetics, Harry Perkins Institute of Medical Research Nedlands, WA 6009, Australia; School of Human Sciences, The University of Western Australia Crawley, WA 6009, Australia.

Nanomedicine : Nanotechnology, Biology, and Medicine
|May 6, 2019
PubMed

Insights

This study introduces novel nanoparticles targeting chemotherapy-resistant triple-negative breast cancer (TNBC). The targeted delivery system effectively reduces tumor growth and induces apoptosis with minimal toxicity, offering a promising new therapeutic strategy.

Area of Science:

  • Oncology
  • Nanotechnology
  • Molecular Biology

Background:

  • Triple-negative breast cancer (TNBC) is aggressive and often develops resistance to chemotherapy, including docetaxel.
  • Overexpression of transcription factor ENGRAILED1 (EN1) is linked to docetaxel resistance in TNBC.

Purpose of the Study:

  • To develop and evaluate a targeted nanoformulation for docetaxel delivery to chemoresistant TNBC.
  • To investigate the efficacy of inhibiting EN1 using interference peptides (EN1-iPeps) conjugated to nanoparticles.

Main Methods:

  • Development of docetaxel-loaded nanoparticles functionalized with EN1-inhibiting peptides (EN1-iPeps) and RGD sequences for enhanced tumor targeting.
  • Assessment of EN1-RGD-iPeps' effect on TNBC cell viability and apoptosis in vitro.
  • Evaluation of nanoparticle internalization via integrins and tumor accumulation after intravenous injection.
  • In vitro and in vivo assessment of the nanoformulation's efficacy in reducing TNBC growth and toxicity.

Main Results:

  • EN1-RGD-iPeps reduced TNBC cell viability and induced apoptosis with minimal impact on normal cells.
  • Nanoparticle uptake in breast cancer cells was mediated by integrins, and intravenous administration increased tumor accumulation.
  • The docetaxel-loaded nanoparticles functionalized with EN1-RGD-iPeps significantly inhibited TNBC growth in vitro and in vivo.
  • The nanoformulation demonstrated no significant toxicity.

Conclusions:

  • Targeted inhibition of EN1 using RGD-functionalized nanoparticles offers a novel therapeutic strategy for chemoresistant TNBC.
  • This nanoformulation shows potential as a safe and effective treatment for TNBC by overcoming chemotherapy resistance.
Keywords:
ENGRAILED 1PeptidesPolymeric nanoparticlesRGD sequencesTriple negative breast cancer

More Related Videos

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy09:01

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

Published on: May 22, 2020

3.5K
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier09:21

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Published on: November 30, 2021

4.4K

Related Concept Videos

Scalar and Vector Triple Products01:06

Scalar and Vector Triple Products

Two vectors can be multiplied using a scalar product or a vector product. The resultant of a scalar product is scalar, while with vector products, the resultant is a vector. These rules of the scalar or vector product between two vectors can be applied to multiple vectors to obtain meaningful combinations. The scalar triple product is the dot product of a vector with the cross product of two vectors.
The scalar triple product is the dot product of a vector with the cross product of two vectors....
4.4K
Peptide Bonds02:43

Peptide Bonds

A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
82.6K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.3K
Positive, Negative, and Zero Work00:58

Positive, Negative, and Zero Work

Work is done on an object when energy is transferred to the object. In other words, work is done when a force acts on a body that undergoes a displacement from one position to another. By definition, the work done by a force is the integral of the force with respect to the displacement along its path. Forces can vary as a function of position, and displacements can occur along various paths between two points. The magnitude of a force multiplied by the cosine of the angle that the force makes...
22.1K
Therapeutic Index01:13

Therapeutic Index

The therapeutic index of a drug is a key parameter in pharmacology that quantifies the relative safety of a drug by calculating the ratio between the dose that causes toxicity in half the population (50%) to the dose that proves to be effective for half the population (50%). It provides a spectrum of doses for a particular drug ranging from effective to potentially toxic. To illustrate, consider an anticoagulant agent like warfarin. It possesses a narrow window within its therapeutic index to...
6.7K
Negative and Cognitive Symptoms of Schizophrenia01:30

Negative and Cognitive Symptoms of Schizophrenia

Negative symptoms of schizophrenia indicate a reduction or absence of typical behaviors and emotional responses found in healthy individuals, while positive symptoms reflect an excess or distortion of normal functioning.
Negative Symptoms
Negative symptoms of schizophrenia manifest as deficits in normal emotional and behavioral functioning, profoundly impacting daily life. Individuals with schizophrenia often display a flat affect, characterized by a near-total absence of emotional expression,...
519