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One step purification of Escherichia coli beta-glucuronidase
Biochimie
|February 1, 1987
Abstract:
beta-glucuronidase was purified by affinity chromatography on thiophenyl-glucuronide coupled to Sepharose. The enzyme was more than 95% pure. This enzyme is a tetramer composed of identical 74 kDa monomers. The amino-terminal sequence determined was: NH2-Met-Leu-Arg-Pro-Val.
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