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Updated: Jan 25, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
Plasma Membrane Proteomic Profile Discovers Macrophage-capping Protein Related to Latent HIV-1
Yujiao Zhang1, Yinzhong Shen1, Lin Yin1
1Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Background:
Due to the persistence of latent HIV-infected cellular reservoirs, HIV virus can not be eradicated completely.
Objective:
To identify proteins related to HIV latency, we performed a subcellular proteomic study in HIV latent cell lines.
Methods:
An established HIV-1 latent cell model (J-Lat Tat-GFP Clone A7 cells, A7 cells) and its parental cell line (Jurkat cells) were used. The plasma membrane (PM) fraction from cultured cells was enriched through aqueous two-phase partition. PM proteins were extracted and then separated using two-dimensional electrophoresis (2DE). Differentially expressed proteins were identified by mass spectrometry, and verified by western blotting.
Results:
Thirteen non-redundant proteins were identified to be differentially expressed in the A7 PM fraction compared to those in the Jurkat PM. Eight had a PM location through Gene Ontology (GO) analysis. A differential protein network of CAPG-ACTR3-CD3D was detected to have interactions with HIV Vpr, Tat, gp160, etc. through STRING software analysis. One of the differential proteins (Macrophage-capping protein (CAPG)) was verified by western blotting to be down- regulated in two cell lines and HIV resting CD4+ T cells negatively selected from patients.
Conclusion:
We identified 13 proteins in A7 compared to Jurkat cells. CAPG may be a potential biomarker related to HIV latency.
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