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Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants
Published on: November 17, 2023
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A quick, easy and cost-effective in planta method to develop direct transformants in wheat
Avijit Tarafdar1, Harinder Vishwakarma1, S Gothandapani1
1National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, 110012 India.
3 Biotech
|May 7, 2019
Summary
Researchers developed herbicide-tolerant wheat using Agrobacterium-mediated transformation. This method successfully integrated the CP4-EPSPS gene into elite Indian wheat, creating a valuable tool for crop improvement and genetic transformation advancements.
Area of Science:
- Agricultural Science
- Plant Biotechnology
- Genetics
Background:
- Wheat (Triticum aestivum) is a global staple crop facing challenges in yield and sustainability.
- Genetic transformation offers a pathway to enhance desirable traits like herbicide tolerance in wheat.
- Developing efficient transformation protocols is crucial for advancing wheat breeding programs.
Purpose of the Study:
- To develop herbicide-tolerant transgenic wheat using an Agrobacterium-mediated in planta transformation method.
- To confirm the stable integration and expression of the CP4-EPSPS gene in elite Indian wheat genotype HD2894.
- To assess the efficiency and potential of the developed transformation system.
Main Methods:
- Agrobacterium-mediated in planta transformation targeting apical meristems of germinated seeds.
- Screening of T1 plants using 1% glyphosate for herbicide tolerance.
- Confirmation of transgene integration via PCR and Southern hybridization.
- Selection of homozygous transgenic lines in the T3 generation based on 100% survival to glyphosate.
Main Results:
- Successful development of herbicide-tolerant transgenic wheat (HD2894) carrying the CP4-EPSPS gene.
- Achieved a transformation efficiency of 3.07%.
- Confirmed stable transgene integration and absence of significant morpho-physiological variations in transgenic lines compared to non-transgenic controls.
Conclusions:
- The Agrobacterium-mediated in planta transformation method is effective for developing herbicide-tolerant wheat.
- The study provides a valuable tool for future genetic improvement and trait introgression in wheat.
- The resulting transgenic wheat lines offer potential for sustainable agriculture with reduced herbicide application.
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