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Published on: November 1, 2019
Potential Use of Hematoxylin-curcumin as Natural Bio-stains for Tissue Sections
Govindarajan Sujatha1, Jayanandan Muruganandhan1, Shankargouda Patil2
1Department of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Thalambur, Chennai, India.
Abstract:
Most bio-stains used in histopathology are synthetic thus pose potential health hazards, especially on long-term exposure. Thus, natural bio-stains which are eco-friendly and biodegradable are the need of the hour. The present manuscript explores the use of curcumin as a potential natural bio-stain for tissue sections. Curcumin is the active component of turmeric and belongs to the Zingiberaceae family.1 Phytochemical analysis of curcumin showed the presence of saponins, tannins, flavonoids, and acidic polyphenolic compounds. Saponins embellish staining property by reducing surface tension.2 Tannins aid in the dying process and flavonoids impart hue. Due to the acidic polyphenolic compounds, curcumin stains the basic tissues including collagen fibers, red blood cells, muscle cells, and cytoplasm.3.
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