Identification of time‑series differentially expressed genes and pathways associated with heart failure...

Area of Science:

• Cardiovascular Biology
• Genomics
• Molecular Medicine

Background:

• Heart failure (HF) following acute myocardial infarction (AMI) presents a significant public health challenge.
• Identifying the molecular mechanisms underlying HF post-AMI is crucial for developing effective therapies.

Purpose of the Study:

• To investigate differentially expressed genes (DEGs) and their functions in HF patients post-AMI.
• To identify potential therapeutic targets for HF following AMI.

Main Methods:

• Analysis of the GSE59867 dataset containing peripheral blood microarray data from HF and non-HF patients post-AMI at four time points.
• Time-series DEG analysis using R Bioconductor, followed by functional enrichment and protein-protein interaction (PPI) network analysis.

Main Results:

• Identified varying numbers of DEGs at different time points post-AMI (108 on admission, 32 at discharge, 41 at 1 month, 19 at 6 months).
• Found four consistently downregulated genes (fatty acid desaturase 2, leucine rich repeat neuronal protein 3, G-protein coupled receptor 15, adenylate kinase 5) across all time points.
• Enrichment analysis highlighted 'inflammatory response', 'immune response', 'toll-like receptor signaling pathway', and 'NF-κβ signaling pathway' as key enriched functions.
• Identified C-X-C motif chemokine ligand 8 and interleukin 1β as hub genes in the PPI network.

Conclusions:

• This study identified candidate DEGs and pathways crucial for HF development post-AMI.
• The findings provide a foundation for developing novel therapeutic strategies targeting HF following AMI.