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Related Concept Videos

Colloids03:22

Colloids

20.9K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
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Colloids and Suspensions01:17

Colloids and Suspensions

3.3K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
3.3K
Colloidal precipitates01:09

Colloidal precipitates

6.0K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
6.0K
Antibody Structure01:10

Antibody Structure

65.4K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.4K
Protein Complex Assembly02:41

Protein Complex Assembly

16.7K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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Spindle Assembly02:50

Spindle Assembly

4.2K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
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Endoscopic Approach for Colloid Cyst Resection
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A Colloid Approach to Self-Assembling Antibodies.

Nicholas Skar-Gislinge1,2, Michela Ronti3, Tommy Garting1

  • 1Physical Chemistry, Department of Chemistry , Lund University , SE-221 00 Lund , Sweden.

Molecular Pharmaceutics
|May 7, 2019
PubMed
Summary
This summary is machine-generated.

Researchers used soft condensed matter physics to address high viscosity in concentrated monoclonal antibody solutions. A patchy colloid model quantitatively describes antibody behavior, aiding pharmaceutical formulation development.

Keywords:
antibodiespatchy colloidsself-assembly

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Synthesis and Characterization of Supramolecular Colloids
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Area of Science:

  • Soft condensed matter physics
  • Biopharmaceutical formulation science
  • Colloid and interface science

Background:

  • Concentrated monoclonal antibody (mAb) solutions are crucial for pharmaceutical formulations.
  • High solution viscosity and protein aggregation are significant challenges in mAb formulation development.
  • Understanding the physical chemistry of concentrated mAbs is essential for therapeutic efficacy and delivery.

Purpose of the Study:

  • To investigate the physical properties of concentrated monoclonal antibody solutions.
  • To develop a theoretical and experimental framework for predicting and mitigating high viscosity.
  • To provide insights into the self-assembly and intermolecular interactions governing mAb behavior.

Main Methods:

  • Utilized a soft condensed matter physics approach combining experimental measurements and theoretical modeling.
  • Employed scattering methods and microrheological experiments to probe structural and dynamic properties.
  • Developed and applied a patchy colloid model accounting for molecular shape and charge distribution.

Main Results:

  • The patchy colloid model quantitatively described the concentration-dependence of osmotic compressibility, collective diffusion, and zero shear viscosity.
  • Successfully disentangled self-assembly effects from intermolecular interactions.
  • Demonstrated the model's ability to predict the behavior of concentrated antibody solutions.

Conclusions:

  • A colloid-inspired patchy model provides a powerful framework for understanding concentrated mAb solutions.
  • This approach offers a quantitative method for assessing formulation strategies, including excipient effects and chemical modifications.
  • The findings facilitate the development of more stable and effective antibody-based therapeutics.