Two-photon excited lasing of Coumarin 307 for lysozyme amyloid fibrils detection

Amyloid fibrils are a well-recognized hallmark of neurodegeneration. A common approach to detect amyloid fibrils is staining with organic molecules and monitoring optical properties using fluorescence spectroscopy. However, the structural diversity of amyloids necessitates new sensitive methods and probes that can be reliably used to characterize them. Here, Coumarin 307 is applied for lysozyme fibrils detection by observation of laser action in the process of two-photon excited stimulated emission. It is shown that the lasing threshold and spectrum significantly depend on the adopted structure (α-helix or β-sheet) of the lysozyme protein, whereas fluorescence spectrum is insensitive to the protein structure. The applications of coherent stimulated emission light that can be emitted deep inside a scattering medium can be particularly promising for imaging and therapeutic purposes in the neurodegeneration field. Two-photon excitation with the near-infrared light, which allows the deepest penetration of tissues, is an important advantage of the method.