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Updated: Jan 25, 2026

Author Spotlight: Studying Behavior of Acanthamoeba to Develop Targeted Strategies for Preventing Acanthamoeba Keratitis
Published on: September 20, 2024
Screening of the pathogen box for the identification of anti-Acanthamoeba agents
Ines Sifaoui1, María Reyes-Batlle2, Atteneri López-Arencibia2
1University Institute of Tropical Diseases and Public Health, Universidad de La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271, la Laguna Tenerife, Canary Islands, Spain; Laboratoire Matériaux-Molécules et Applications, IPEST, B.P 51 2070, La Marsa, University of Carthage, Tunisia.
Abstract:
Acanthamoeba are free living amoeba that have been isolated from different environments like soil, water, air dust. Moreover, they are also able to act as opportunist pathogens, mainly causing a fatal encephalitis and also keratitis in both human and animals. This study was aimed to evaluate the activity of the Medicines for Malaria Venture (MMV) compounds against the trophozoite stage of Acanthamoeba castellanii Neff. Sixteen compounds showed ≥90% inhibition of parasite growth in the initial screen (10 μM). Those set were further evaluated to determine the inhibitor concentration that inhibit the 50% of the initial population and cytotoxicity against murine macrophages. Among the compounds included in the pathogen box, pentamidine and posaconazole were the most effective against this parasite with an of IC50 of 0.567 ± 0.04 and 0.630 ± 0.11, respectively.
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