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Techniques to Induce and Quantify Cellular Senescence
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Aging and Senescence in Canine Testes.

Sophie E Merz1, Robert Klopfleisch1, Angele Breithaupt1

  • 11 Institute of Veterinary Pathology, Freie Universität, Berlin, Germany.

Veterinary Pathology
|May 8, 2019
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Cellular senescence markers increased with age in canine testes, but did not correlate with testicular tumors. Further research is needed to understand senescence in the tumor microenvironment.

Keywords:
dogsimmunohistochemistryp21senescencetestistumor microenvironmentγH2AX

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Area of Science:

  • Veterinary Pathology
  • Cellular Biology
  • Aging Research

Background:

  • Cellular senescence, the process of cells ceasing to divide, is linked to aging and tumor development.
  • Tissue studies on senescent cells are limited, especially in canine reproductive organs.
  • Dogs exhibit age-related diseases, but cellular senescence in their testes is unconfirmed.

Purpose of the Study:

  • To investigate the accumulation of senescent cells in aging canine testes.
  • To determine if senescent cells are present in the microenvironment of canine testicular tumors.
  • To correlate age-dependent senescence with morphological changes in canine testes.

Main Methods:

  • Examined testes from young and old dogs for senescence markers p21 and γH2AX.
  • Quantified marker expression in testicular fibroblasts and Leydig cells.
  • Assessed age-related morphological changes, including Leydig cell number and spermatogenesis.
  • Evaluated senescence markers in the microenvironment of testicular tumors.

Main Results:

  • A significant age-dependent increase in p21-expressing cells was found in testicular fibroblasts and Leydig cells.
  • p21-expressing cells remained rare, and γH2AX-positive cells did not increase with age.
  • Senescence markers were infrequent in the microenvironment of testicular tumors.
  • Age-related morphological changes included increased Leydig cells and decreased spermatogenesis, but not collagen or tubular diameter.

Conclusions:

  • While some senescence markers increase with age in canine testes, they do not appear to correlate with testicular tumor development.
  • The role of the microenvironment in senescence, particularly concerning tumors, requires further investigation.
  • This study provides foundational data on senescence in canine testes, highlighting a discrepancy with in vitro findings.