Related Experiment Video
Updated: Jan 25, 2026

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow
Published on: November 11, 2010
Visualization of in vitro deep blood vessels using principal component analysis based laser speckle imaging
Jose Angel Arias-Cruz1, Roger Chiu2, Hayde Peregrina-Barreto1
1Instituto Nacional de Astrofísica, Óptica y Electrónica, Luis Enrique Erro 1, Tonantzintla, Puebla, México.
Abstract:
Visualization of blood vessels is a fundamental task in the evaluation of the health and biological integrity of tissue. Laser speckle contrast imaging (LSCI) is a non-invasive technique to determine the blood flow in superficial or exposed vasculature. However, the high scattering of biological tissue hinders the visualization of those structures. In this paper, we propose the use of principal component analysis (PCA) in combination with LSCI to improve the visualization of deep blood vessels by selecting the most significant principal components. This analysis was applied to in vitro samples, and our results demonstrate that this approach allows for the visualization and localization of blood vessels as deep as 1000 μm.
More Related Videos
07:20A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
Published on: August 30, 2017
06:24Paired Cisterna Magna Nanoinjection and Laser Speckle Contrast Imaging Assay to Study Cerebral Blood Flow Regulation In Vivo
Published on: July 8, 2025
Related Concept Videos
Structure of Blood Vessels
Anatomy of Blood Vessels
Arteries
Arteries circulate oxygenated blood from the heart, except the pulmonary artery, which transports deoxygenated blood to the lungs. Large arteries, such as the aorta,...
Overview of Blood Vessels
Arteries and Arterioles: Arteries are muscular and elastic vessels that primarily carry oxygenated blood from the heart to body tissues, except for the pulmonary artery, which carries deoxygenated blood. They have thick walls to withstand high pressure and contain a layer of muscle tissue, allowing them to expand or contract as...
Development of Blood Vessels
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
Drug Binding to Blood Components
HSA is the most abundant plasma protein and is vital in drug binding. It contains distinct drug-binding sites, with different drugs exhibiting affinity for specific sites. There are three main drug-binding domains for HSA: sites I, II, and III. These domains are...
Principal Stresses in a Beam
Analyzing principal stresses is crucial, especially in...