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Identification and characterization of a secreted M28 aminopeptidase protein in Acanthamoeba

Jian-Ming Huang1, Yao-Tsung Chang2, Min-Hsiu Shih3

  • 1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

Parasitology Research
|May 9, 2019
PubMed

Insights

Acanthamoeba

Area of Science:

  • Microbiology
  • Immunology
  • Parasitology

Background:

  • Acanthamoeba is a protozoan found in soil and water.
  • It causes severe human diseases like keratitis and encephalitis.
  • Understanding Acanthamoeba's pathogenicity is crucial.

Purpose of the Study:

  • Identify novel Acanthamoeba virulence factors.
  • Investigate the role of M28 aminopeptidase (M28AP) in immune evasion.
  • Explore M28AP's impact on human innate immunity.

Main Methods:

  • Comparative analysis of Acanthamoeba isolates.
  • Generation of an M28AP mutant strain using CRISPR/Cas9.
  • Degradation assays using human complement proteins (C3b, iC3b).

Main Results:

  • A novel secreted protein, M28 aminopeptidase (M28AP), was identified.
  • M28AP degrades human complement proteins C3b and iC3b.
  • M28AP plays a role in evading the human innate immune response.

Conclusions:

  • M28AP is a significant factor in Acanthamoeba pathogenicity.
  • M28AP contributes to Acanthamoeba's ability to evade innate immunity.
  • M28AP represents a potential therapeutic target for Acanthamoeba infections.
Keywords:
AcanthamoebaCRISPRComplement proteinM28APSecreted protein

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