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Related Experiment Video

Updated: Jan 25, 2026

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

Published on: January 20, 2023

Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model.

Fateme Vafashoar¹, Kazem Mousavizadeh², Hadi Poormoghim³

¹Department of Immunology, Iran University of Medical Sciences, Tehran, Iran. vafashoarf@yahoo.com.

Iranian Journal of Allergy, Asthma, and Immunology

|May 9, 2019

Summary

This study shows that bleomycin induces fibrosis in mice, increasing gelatinase activity in lung and skin tissues. Matrix metalloproteinase 9 (MMP9) and MMP2 levels rise in fibrotic skin, while MMP9 increases in fibrotic lungs.

Keywords:

Bleomycin Gelatinase Matrix metalloproteinase 2 Matrix metalloproteinase 9 Systemic scleroderma

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Last Updated: Jan 25, 2026

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

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Published on: January 20, 2023

Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin

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Published on: April 30, 2019

Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment

Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment

Published on: June 28, 2018

Area of Science:

Immunology
Biochemistry
Pathology

Background:

Systemic sclerosis is a fibrotic autoimmune disease characterized by extracellular matrix remodeling.
Organ dysfunction arises from aberrant extracellular matrix remodeling in systemic sclerosis.

Purpose of the Study:

To investigate the expression and activity of gelatinases in a mouse model of systemic sclerosis.
To evaluate the role of matrix metalloproteinases (MMPs) in bleomycin-induced fibrosis.

Main Methods:

A mouse model of systemic sclerosis was induced using bleomycin injection.
Fibrosis was assessed via trichrome staining, hydroxyproline measurement, and alpha-smooth muscle actin (α-SMA) detection.
Gelatinolytic activity of MMP2 and MMP9 was measured using gelatin zymography in lung and skin tissues.

Main Results:

Bleomycin administration successfully induced fibrosis in mouse skin and lung tissues.
Pro and active forms of MMP9 were significantly increased in fibrotic lung tissues (p<0.05 and p<0.01).
Both pro and active forms of MMP2 and MMP9 were elevated in fibrotic skin tissues (p<0.05).
MMP2 activity remained unchanged in fibrotic lung tissues.

Conclusions:

Gelatinase expression, particularly MMP9 and MMP2, is altered in fibrotic tissues during bleomycin-induced systemic sclerosis.
The increase in pro and active gelatinase forms differs between skin and lung tissues.
These findings highlight the differential role of MMPs in the pathogenesis of systemic sclerosis affecting different organs.