Related Experiment Video
Updated: Jan 25, 2026

Preparation of Viral DNA from Nucleocapsids
Published on: August 16, 2011
Visualization of viral DNA assembly using nonfluorescent staining
E M Karalova1, L H Hakobyan1, M R Tatoyan2
1a Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS , Yerevan , Armenia.
Abstract:
We present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilitates their use in everyday experiments. Methyl green is most promising for routine detection of viral DNA assemblies in the cytoplasm; the procedure enables ready detection of viral DNA accumulation in the cytoplasm.
Related Concept Videos
Viral Structure
Viral Recombination
Complementary DNA
From DNA to Protein
Protein Complex Assembly
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Viral Mutations

