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[Citron Rho-interacting serine/threonine kinase knockdown suppresses prostate cancer cell proliferation and

Chen Haiping1,2, Xiang Qi2, Liu Dawei2

  • 1Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|May 10, 2019
PubMed
Abstract

Insights

Citron Rho-interacting serine/threonine kinase (CIT) promotes prostate cancer (PCa) progression by influencing cell proliferation, migration, and invasion. Targeting CIT may offer a new therapeutic strategy for PCa by modulating the Hippo-YAP pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • Citron Rho-interacting serine/threonine kinase (CIT) is an identified oncogene in various cancers.
  • The specific role of CIT in prostate cancer (PCa) progression and its underlying mechanisms are not well understood.

Purpose of the Study:

  • To investigate the biological functions and clinical significance of CIT in prostate cancer.
  • To explore the potential of CIT as a therapeutic target for PCa.

Main Methods:

  • Analysis of CIT expression in PCa tissues utilizing TCGA and MSKCC datasets.
  • Assessment of CIT's clinical correlations with staging, metastasis, Gleason score, and PSA levels.
  • In vitro studies involving RNA interference-mediated CIT silencing in PC-3 cells to evaluate proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and Hippo-Yap signaling pathway activity via Western blotting.

Main Results:

  • Significantly elevated CIT expression was observed in PCa tissues.
  • Higher CIT expression correlated with advanced N stage, distant metastasis, higher Gleason score, and elevated PSA levels.
  • CIT knockdown in PC-3 cells suppressed proliferation, migration, and invasion, reversed EMT, and reduced YAP expression and activity.

Conclusions:

  • CIT acts as an oncogene in prostate cancer, potentially by regulating the Hippo-YAP signaling pathway.
  • CIT represents a promising candidate for targeted therapy in PCa treatment.
Keywords:
Citron Rho-interacting serine/threonine kinaseHippo-YAP pathwayinvasionmigrationprostate cancer

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