Related Experiment Video

Updated: Jan 25, 2026

Robot Assisted Distal Pancreatectomy with Celiac Axis Resection DP-CAR for Pancreatic Cancer: Surgical Planning and Technique
13:56

Robot Assisted Distal Pancreatectomy with Celiac Axis Resection DP-CAR for Pancreatic Cancer: Surgical Planning and Technique

Published on: August 14, 2021

7.2K

Analysis of DLL3 and ASCL1 in Surgically Resected Small Cell Lung Cancer (HOT1702)

Megumi Furuta1, Jun Sakakibara-Konishi2, Hajime Kikuchi3

  • 1Department of Respiratory Medicine, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

The Oncologist
|May 10, 2019
PubMed
Abstract

Insights

Delta-like protein 3 (DLL3) and ASCL1 are highly expressed in small cell lung cancer (SCLC). These proteins may serve as therapeutic targets for SCLC treatment, including with Rova-T.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • Delta-like protein 3 (DLL3) is a Notch ligand implicated in small cell lung cancer (SCLC) tumorigenesis.
  • ASCL1 is a transcription factor involved in pulmonary neuroendocrine cell development and regulates DLL3 expression.
  • Rovalpituzumab tesirine (Rova-T) is a novel DLL3-targeted antibody-drug conjugate for SCLC treatment.

Purpose of the Study:

  • To investigate the expression of DLL3 and ASCL1 in surgically resected SCLC samples.
  • To determine the correlation between DLL3 and ASCL1 expression and clinicopathological features.
  • To explore the potential of DLL3 and ASCL1 as therapeutic targets in SCLC.

Main Methods:

  • Analysis of 95 surgically resected SCLC samples using immunohistochemical staining.
  • Correlation analysis between DLL3/ASCL1 expression and patient clinicopathological features.

Main Results:

  • DLL3 expression was detected in 83% of samples, with high expression in 47%.
  • ASCL1 expression was observed in 64% of samples.
  • A positive correlation was found between DLL3 and ASCL1 expression; neither was associated with survival, but DLL3 was more prevalent in advanced disease.

Conclusions:

  • DLL3 and ASCL1 are highly expressed in surgically resected SCLC.
  • DLL3 and ASCL1 represent potential therapeutic targets for SCLC, including early-stage disease and Rova-T treatment.
Keywords:
Achaete‐scute homolog‐1Delta‐like protein 3ImmunohistochemistrySmall cell lung cancerSurgery

More Related Videos

An Orthotopic Resectional Mouse Model of Pancreatic Cancer07:17

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

Published on: September 24, 2020

12.3K
Surgical Procedures and Methodology for a Preclinical Murine Model of De Novo Mammary Cancer Metastasis08:41

Surgical Procedures and Methodology for a Preclinical Murine Model of De Novo Mammary Cancer Metastasis

Published on: July 29, 2017

14.6K

Related Concept Videos

Cancer Survival Analysis01:21

Cancer Survival Analysis

Cancer survival analysis focuses on quantifying and interpreting the time from a key starting point, such as diagnosis or the initiation of treatment, to a specific endpoint, such as remission or death. This analysis provides critical insights into treatment effectiveness and factors that influence patient outcomes, helping to shape clinical decisions and guide prognostic evaluations. A cornerstone of oncology research, survival analysis tackles the challenges of skewed, non-normally...
659
Lung Capacity01:47

Lung Capacity

The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.2K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.2K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
7.0K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.9K
Pleura of the Lungs01:13

Pleura of the Lungs

The lungs are nestled in a cavity, shielded by the pleura. The pleura, a form of serous membrane, wraps around each lung. This membrane arrangement consists of two layers: the visceral and parietal pleurae. The visceral pleura lines the surface of the lungIn contrast, the parietal pleura is the outer layer and contacts to the thoracic wall, the mediastinum, and the diaphragm. The hilum is the point of connection between the visceral and parietal layers. The space between the parietal and...
6.9K