Related Experiment Video

Updated: Jan 25, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.8K

A TaqMan Probe-Based Real-Time PCR Assay for the Rapid Identification of the Emerging Multidrug-Resistant Pathogen

Amorce Lima1, Raymond Widen2, Grant Vestal2

  • 1Esoteric Testing/R&D Laboratory, Tampa General Hospital, Tampa, Florida, USA alima@tgh.org.

Journal of Clinical Microbiology
|May 10, 2019
PubMed

Insights

A new real-time PCR assay accurately identifies Candida auris, a dangerous multidrug-resistant fungus. This rapid diagnostic tool helps prevent misidentification and improves patient care in intensive care units (ICUs).

Area of Science:

  • Clinical Microbiology
  • Molecular Diagnostics
  • Infectious Diseases

Background:

  • Candida auris is an emerging, multidrug-resistant fungal pathogen causing severe nosocomial infections, particularly in intensive care unit (ICU) patients.
  • Current yeast identification methods, relying on phenotypic testing, frequently misidentify C. auris, hindering timely and appropriate treatment.
  • Accurate and rapid diagnostics are crucial for managing C. auris outbreaks and improving patient outcomes.

Purpose of the Study:

  • To develop and validate a novel TaqMan-based real-time PCR assay for the accurate and rapid detection of Candida auris.
  • To evaluate the assay's performance on the BD Max platform using both cultured isolates and direct clinical specimens.
  • To provide a reliable diagnostic tool to overcome the limitations of current phenotypic identification methods.

Main Methods:

  • Development of a TaqMan-based real-time PCR assay targeting the ribosomal DNA (rDNA) region of Candida auris.
  • Validation of the assay on the BD Max automated platform.
  • Testing of the assay's sensitivity, specificity, and reproducibility using various samples, including clinical specimens.

Main Results:

  • The developed real-time PCR assay demonstrated high specificity and reproducibility.
  • The assay achieved a high level of sensitivity, detecting as few as 1 Candida auris Colony Forming Unit (CFU) per reaction.
  • The diagnostic test provided accurate identification of C. auris within 3 hours.

Conclusions:

  • The validated TaqMan-based real-time PCR assay offers a rapid, accurate, and reliable method for identifying Candida auris.
  • This molecular diagnostic approach effectively addresses the misidentification issues associated with conventional phenotypic methods.
  • The assay has the potential to significantly improve the management of C. auris infections in healthcare settings.
Keywords:
BD MaxCandida aurisTaqMan probereal-time PCR

More Related Videos

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

17.9K
Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis08:48

Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis

Published on: March 19, 2019

7.4K

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.9K
Emerging Adulthood01:27

Emerging Adulthood

Jeffrey Arnett's concept of emerging adulthood offers a framework to understand the unique developmental stage between adolescence and full-fledged adulthood, generally from ages 18 to 25. This period is marked by extensive exploration and shifts in identity, relationships, and career choices, a process known in psychology as role experimentation. Emerging adulthood reflects the evolving cultural expectations surrounding adulthood and the dynamic process of personal transformation during...
653
PCR01:32

PCR

Overview
237.2K
Introduction Cardiac Emergencies01:30

Introduction Cardiac Emergencies

Cardiac emergencies are critical situations involving the heart that require immediate medical intervention to prevent severe complications or death. These emergencies often arise from underlying heart conditions that impair the heart's ability to function correctly.Types of Cardiac EmergenciesThe most common types of cardiac emergencies include Acute Coronary Syndrome (ACS), myocardial infarction (MI), cardiac arrest, and heart failure.Acute Coronary Syndrome (ACS)Acute Coronary Syndrome (ACS)...
317
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.4K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
5.7K