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Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities
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The Chicken Embryo as a Model in Developmental Toxicology
Michael R Stark1, Micah M Ross2
1Department of Physiology and Developmental Biology, College of Life Sciences, Brigham Young University, Provo, UT, USA. michael_stark@byu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|May 10, 2019
Summary
Chicken embryos offer a robust model for assessing developmental toxicity. This guide details methods for exposing embryos to teratogens and analyzing their effects on early development.
Area of Science:
- Developmental toxicology
- Embryology
- Pharmacology
Background:
- The chicken embryo is a widely used model organism in toxicological studies.
- Early developmental stages are critical and susceptible to environmental insults.
- Understanding teratogenic effects is crucial for human health and safety.
Purpose of the Study:
- To provide detailed protocols for using the chicken embryo model in toxicology.
- To outline methods for embryonic collection and toxicant exposure.
- To describe techniques for analyzing cellular responses to teratogens.
Main Methods:
- Embryonic collection and preparation (e.g., EC culture, Cornish Pasty methods).
- Exposure of chicken embryos to teratogenic compounds.
- Analysis of cellular health using LysoTracker staining.
- Assessment of oxidative stress via glutathione redox potential.
- Measurement of reactive oxygen species using 2',7'-dichlorodihydrofluorescein diacetate.
Main Results:
- Established protocols for reliable embryonic exposure and analysis.
- Demonstrated feasibility of assessing teratogenic compound effects.
- Provided a framework for evaluating cellular damage and stress responses.
Conclusions:
- The chicken embryo model is effective for studying teratogenic compound effects.
- Detailed protocols facilitate reproducible toxicological assessments.
- This approach aids in understanding developmental toxicity mechanisms.
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