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Assessment of Histiotrophic Nutrition Using Fluorescent Probes
1Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI, USA. charris@umich.edu.
Abstract:
Histiotrophic nutrition is a process whereby the rodent visceral yolk sac (VYS) internalizes exogenous macromolecules, degrades them, and sends the degradation products to the embryo. Quantification and visualization of histiotrophic nutrition can be accomplished using fluorescent tracer molecules such as fluorescein isothiocyanate-conjugated albumin (FITC-albumin). The methods are simple and can provide complimentary functional and structural information in studies of the effects of embryotoxicants on visceral yolk sac function.
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