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The Effect of Boron-Containing Nano-Hydroxyapatite on Bone Cells
Merve Gizer1, Sevil Köse2, Beren Karaosmanoglu3
1Graduate School of Science and Engineering, Department of Bioengineering, Hacettepe University, Ankara, Turkey.
Biological Trace Element Research
|May 10, 2019
Summary
Boron-loaded hydroxyapatite composites promote bone healing by enhancing mesenchymal stem cell differentiation and proliferation. This biomaterial offers a controlled release of boron, supporting extracellular matrix construction and potentially treating bone injuries.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Bone Biology
Background:
- Metabolic diseases and injuries impair bone structure and regeneration.
- Trace elements and hydroxyapatite are crucial for bone extracellular matrix (ECM) renewal.
- Developing effective biomaterials for bone repair is essential.
Purpose of the Study:
- To investigate the potential of boron-loaded nanometer-sized hydroxyapatite (nHA) composites for bone regeneration.
- To assess the controlled boron release and its impact on SaOS-2 osteoblasts and human bone marrow-derived mesenchymal stem cells (hMSCs).
- To elucidate the molecular mechanisms underlying the therapeutic effects of boron-nHA composites.
Main Methods:
- Synthesized boron-loaded nHA composites and characterized boron release kinetics using ICP-MS.
- Evaluated cell proliferation of SaOS-2 and hMSCs using WST-1 assay.
- Assessed osteogenic differentiation via alkaline phosphatase (ALP) activity.
- Performed transcriptomic analysis to identify affected signaling pathways.
Main Results:
- Boron release from nHA composites occurred rapidly initially, followed by sustained low-dose release.
- Boron-nHA composites enhanced hMSC proliferation at high doses and modulated osteoblastic cell proliferation.
- Osteogenic differentiation of hMSCs was significantly increased by boron-nHA composites, evidenced by higher ALP activity.
- Transcriptomic analysis revealed that effective doses of boron-nHA composites influence Wnt, TGF-β, and stress response pathways.
Conclusions:
- Boron-loaded nHA composites demonstrate potential as osteoconductive biomaterials for bone repair.
- Controlled boron release from nHA composites supports osteogenesis and ECM construction.
- Further studies are needed to define the safe therapeutic window and validate the regenerative effects.
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