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Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions
1National Institute of Health, Centre for the Control and Evaluation of Medicines, Rome, Italy. roberto.cirilli@iss.it.
Abstract:
In contrast to achiral hydrophilic interaction liquid chromatography (HILIC), which is a popular and largely applied technique to analyze polar compounds such as pharmaceuticals, metabolites, proteins, peptides, amino acids, oligonucleotides, and carbohydrates, the introduction of the HILIC concept in enantioselective chromatography has been relatively recent and scarcely debated. In this chapter, the HILIC enantioseparations carried out on polysaccharide-based chiral stationary phases are grouped and discussed. Another objective of this chapter is to provide a comprehensive overview and insight into the experimental conditions needed to operate under HILIC mode. Finally, to stimulate and facilitate the application of this chromatographic technique, a detailed experimental protocol of a chiral resolution on a chlorinated cellulose-based chiral stationary phase under HILIC conditions is described.
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