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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
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Chiral Capillary Electrophoresis-Mass Spectrometry
María Castro-Puyana1, María Luisa Marina2
1Departamento de Química Analítica, Química Física e Ingeniería Química, Facultad de Ciencias, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|May 10, 2019
Summary
Capillary electrophoresis-mass spectrometry (CE-MS) offers sensitive chiral separations for amino acids in biological fluids. Three distinct CE-MS methods are presented, showcasing advanced techniques for enantioseparation analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biochemistry
Background:
- Capillary electrophoresis (CE) is a powerful technique for chiral separations.
- Hyphenation with mass spectrometry (MS) enhances sensitivity and provides structural information.
- CE-MS combines the strengths of both techniques for sensitive enantioseparations.
Purpose of the Study:
- To describe three distinct CE-MS methodologies for chiral analysis of amino acids in biological fluids.
- To showcase different approaches for achieving sensitive enantioseparations using CE-MS.
Main Methods:
- Partial filling technique to prevent cyclodextrin entry into the MS source.
- Direct coupling of capillary electrokinetic chromatography-MS (EKC-MS) with native cyclodextrins.
- Formation of stable diastereomers for separation in an achiral environment.
Main Results:
- Demonstration of effective chiral separation of amino acids in biological samples using CE-MS.
- Successful implementation of three different strategies for enantioseparation.
- Validation of CE-MS as a robust tool for sensitive chiral analysis.
Conclusions:
- CE-MS is an outstanding combination for sensitive enantioseparations.
- Diverse CE-MS approaches enable effective chiral analysis of amino acids.
- These methods are applicable to biological fluid analysis.
Keywords:
Capillary electrophoresisChiralCyclodextrinsDiastereomeric separationEnantiomeric separationMass spectrometryPartial filling techniqueMore Related Videos
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