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Published on: July 18, 2014
Comparative proteomic analysis of plasma of children with congenital heart disease
Qiong Huang1,2, Zhi Geng3, Tao Chen1
1The First Affiliated Hospital, Nanjing Medical University, Nanjing, P. R. China.
Insights
Researchers identified potential biomarkers for ventricular septal defect (VSD) in children. Proteomic analysis revealed significant protein changes in VSD patients, with ALDOB and Tβ4 showing promise for diagnosis.
Area of Science:
- Biochemistry
- Proteomics
- Pediatric Cardiology
Background:
- Congenital heart disease (CHD) represents a significant category of birth defects.
- Ventricular septal defect (VSD) is a common type of CHD, necessitating improved diagnostic methods.
Purpose of the Study:
- To compare plasma protein abundance between children with VSD and healthy controls.
- To identify potential protein biomarkers for VSD diagnosis.
Main Methods:
- Tandem mass tags (TMT) label-based quantitative proteomic analysis was performed on plasma samples.
- Differentially abundant proteins were identified and validated using Enzyme-Linked Immunosorbent Assay (ELISA).
Main Results:
- A total of 66 proteins showed significant differential abundance between VSD patients and controls.
- Proteins were associated with pathways including platelet activation, metabolism, and complement/coagulation cascades.
- Fructose-bisphosphate aldolase B (ALDOB) and thymosin beta-4 (Tβ4) demonstrated high diagnostic potential based on receiver operating characteristic (ROC) curve analysis.
Conclusions:
- ALDOB and Tβ4 are identified as potential biomarkers for VSD.
- Further research is warranted to validate ALDOB and Tβ4 as diagnostic tools for VSD.
Abstract:
Congenital heart disease is one of the largest class of birth defects. Eight subjects with ventricular septal defect (VSD, a kind of congenital heart disease) and 11 health children were enrolled in tandem mass tags label-based quantitative proteomic analysis to compare plasma proteins differentially abundance. A total of 66 proteins were significantly upregulated or downregulated in VSD patients compared with healthy children. These proteins were involved in pathways linked to platelet activation, fructose and mannose metabolism, complement and coagulation cascades, glycolysis/gluconeogenesis, regulation of actin cytoskeleton, and carbon metabolism. The amount of ten proteins changed significantly (p < 0.05) in newly recruited 30 VSD compared with 15 control children, which were validated by ELISA. The areas under the receiver operating characteristic curve values of fructose-bisphosphate aldolase B (ALDOB) and thymosin beta-4 (Tβ4) were higher than those of other candidate proteins. ALDOB and Tβ4 might be potential biomarkers applied for identifying VSD in the further works.
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