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Developmental expression, co-localization and genetic interaction of exocyst component Sec15 with Rab11 during
1Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221 005, India; Department of Zoology, The University of Burdwan, Golapbag, Burdwan, 713104, India.
Drosophila Sec15 (DSec15) protein and transcript are maternally loaded and widely expressed during development. Sec15 interacts with Rab11, impacting blister formation in Drosophila wing development.
Area of Science:
- Cell Biology
- Developmental Biology
- Genetics
Background:
- Sec15 is a component of the evolutionarily conserved octomeric exocyst complex.
- Sec15 interacts with GTP-bound Rab11 and is involved in secretion and intracellular vesicle transport.
Purpose of the Study:
- To investigate the expression patterns of Drosophila Sec15 (DSec15) transcript and protein during Drosophila development.
- To elucidate the interaction between Sec15 and Rab11 in Drosophila development.
Main Methods:
- Analysis of DSec15 transcript and protein expression patterns during embryonic and larval stages.
- Co-localization studies of Sec15 and Rab11.
- Genetic approach to study Sec15-Rab11 interaction in wing development.
Main Results:
- DSec15 transcript and protein show maternal loading and distinct expression patterns in various embryonic and larval tissues, including imaginal discs.
- Sec15 co-localizes with Rab11 throughout Drosophila development.
- Sec15 interacts with Rab11 to cause blister formation during Drosophila wing development.
Conclusions:
- DSec15 plays a significant role in Drosophila development, with expression correlating with key developmental processes.
- The interaction between Sec15 and Rab11 is crucial for normal Drosophila development, particularly in wing morphogenesis.
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