Combined microbial and isotopic signature approach to identify nitrate sources and transformation processes in...

Nitrate (NO₃^-) pollution is a serious problem worldwide. Identification of NO₃^- sources and transformation processes in aquifers is a key step in effectively controlling and mitigating NO₃^- contamination. In this study, hydrochemical, microbial, and dual isotopic approaches were integrated to elucidate the sources and processes influencing NO₃^- contamination in the Pearl River Delta, China. The results showed a severe NO₃^- contamination, with 75% of the samples having NO₃^--N concentrations above the WHO standard of 10 mg L^-1. The δ¹⁵NNO₃^- and δ¹⁸ONO₃^- values and a multivariate statistical analysis of hydrochemical data both revealed that manure and sewage were mainly responsible for NO₃^- contamination. Biological indicators further demonstrated that, manure and sewage had greater impacts on groundwater quality during the rainy season than during the dry season. Based on the significant relationships of δ¹⁵NNO₃^- and δ¹⁸ONO₃^- with the logarithmic NO₃^- concentration (Ln(NO₃^-)), denitrification was confirmed to occur in the discharge zone during the rainy season. Proteobacteria, Bacteroidetes, and Planctomycetes were identified as the dominant phyla, and Dechloromonas, Flavobacterium, and Nitrospira were dominant among the denitrifying bacteria in groundwater. The abundance of denitrifying bacteria had significant positive correlations with δ¹⁵NNO₃^- and NO₂^--N during the rainy season, further confirming the occurrence of denitrification during the rainy season. This study showed that dual isotope techniques combined with microbial data can be a powerful tool for identifying the sources and microbial processes affecting NO₃^- in groundwater. Moreover, the results can provide useful insights for environmental managers to verify groundwater pollution and better apply remediation solutions.