Related Experiment Video
Updated: Jan 25, 2026

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
Published on: September 22, 2019
Survival and Growth in vitro of Paramoeba perurans Populations Cultured Under Different Salinities and Temperatures
Catherine Collins1, Malcolm Hall1, Mark J Fordyce1
1Marine Scotland, 375 Victoria Road, Aberdeen AB11 9DB, United Kingdom.
Abstract:
Growth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15day period. Optimal population growth, under the experimental conditions, was observed at 15°C and a salinity of 35‰, with amoebae populations doubling every 14h. Positive P. perurans populations growth was observed at 15°C between salinities of above 20‰ and 50‰, and at 8°C, 11°C and 18°C at salinities between 25‰ and 50‰, 50‰ being the maximum salinity tested. Amoebae numbers were sustained at 4°C. Therefore, lower temperature and salinity thresholds for P. perurans population growth lie between 4 to 8°C, and salinities of 20 to 25‰, respectively. Upper limits were not determined in this study. The populations remained relatively stable at 4°C and 2°C at permissive salinities with respect to numbers of viable amoebae over the 15day exposure period.
Related Concept Videos
Population Growth
Factors Influencing Microbial Growth: Temperature
Physical Methods for Controlling Microbial Growth: Temperature
Conservation of Small Populations
Survival Tree
Building a Survival Tree
Constructing a...
Survival Curves
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...

