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Updated: Jan 25, 2026

Listeria monocytogenes Infection of the Brain
Published on: October 2, 2018
Sensitivity and Resistance of Listeria monocytogenes ATCC 19115, Scott A, and UAL500 to Nisin
Linda J Harris1,2,3, Henry P Fleming1,2, Todd R Klaenhammer2,3,4
1Food Fermentation Laboratory, U.S. Department of Agriculture, Agricultural Research Service, and North Carolina Agricultural Research Service.
Abstract:
Listeria monocytogenes ATCC 19115, Scott A, and UAL500 were evaluated for sensitivity to nisin (0 to 50 μg/ml) using a direct plating method. Nisin (10 μg/ml) decreased an initial population of L. monocytogenes (109 CFU per ml) by 6- to 7-log cycles. Sensitivity to nisin was enhanced by addition of 2% NaCl or by reduction of the medium pH from 6.5 to 5.5 with either hydrochloric or lactic acid. Mutants resistant to 50 μg/ml nisin were detected at frequencies of 10-6 to 10-8. Nisin-resistant L. monocytogenes mutants should be expected to arise when nisin is used as an antimicrobial in food systems.
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