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Updated: Jan 25, 2026

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Using Thermography to Confirm Genotypic Variation for Drought Response in Maize
Raphael A C N Casari1, Dayane S Paiva2, Vivianny N B Silva3,4
1Embrapa Agroenergia, Parque Estação Biológica (PqEB), Avenida W3 Norte (Final), Brasília 70.770-901, DF, Brazil. casari.raphael@gmail.com.
Thermography effectively screens maize genotypes for drought tolerance, mirroring field classifications. However, canopy temperature doesn't fully predict physiological responses under drought, impacting yield correlations.
Area of Science:
- Plant physiology and genetics
- Agricultural science
- Drought stress response
Background:
- Thermography is a viable tool for assessing plant drought tolerance.
- Previous studies linked thermography to gas exchange, biomass, and yield.
- Maize genotype classification for drought tolerance is often field-based, primarily using grain yield.
Purpose of the Study:
- To evaluate thermography for phenotyping contrasting maize genotypes.
- To compare thermography-based classification with established field classifications for drought tolerance.
- To determine if canopy temperature accurately reflects physiological responses under drought.
Main Methods:
- Phenotyping contrasting maize genotypes using thermography.
- Evaluating gas exchange, daily water consumption, leaf relative water content, biomass, and grain yield.
- Comparing thermography-based screening with field selection criteria (grain yield).
Main Results:
- Thermography-based screening of maize genotypes yielded results similar to traditional field methods.
- Canopy temperature partially correlated with gas exchange and water consumption under drought.
- Plant structural and physiological changes under drought influenced energy absorption and dissipation.
Conclusions:
- Thermography is a useful tool for classifying maize genotypes for drought tolerance.
- Canopy temperature alone may not fully capture complex physiological responses to drought stress.
- The relationship between cooler canopies and higher yield under drought is not always direct.
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