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Further evidence supporting the concurrent influence of aflatoxin and manganese
Abstract:
Trace elements, including manganese may afford protection from deleterious effects of aflatoxin. Young male Fischer rats received ip injections of aflatoxin B1 (AFB1) in dimethyl sulfoxide (DMSO), 1 mg/kg, 2 mg/kg or 4 mg/kg. Control groups received DMSO ip or no injection. All animals were intubated with 3 microCi of [54Mn]-MnCl2 12 hr post-injection. Sacrifice occurred 72 hr after gavage of the radiolabel. All tested levels of AFB1 affected the loss of total body radioactivity. This response was observed within 12 hr when toxin-treated groups excreted almost 4 times more counts than controls. From 12-36 hr following radiolabel administration, AFB1 appeared to enhance excretion; by 72 hr, toxin-treated animals (especially those receiving higher doses) appeared to conserve the metal. Aflatoxicosis manifested itself through reduced body weight gain. The data provide support evidence that Mn and AFB1 biointeract.
Insights
Manganese (Mn) may protect against aflatoxin B1 (AFB1) toxicity. Studies show AFB1 alters Mn excretion and retention in rats, suggesting a significant biointeraction between these substances.
Area of Science:
- Toxicology
- Biochemistry
- Nutritional Science
Background:
- Aflatoxin B1 (AFB1) is a toxic compound produced by Aspergillus fungi.
- Trace elements, such as manganese (Mn), play crucial roles in cellular processes and may influence detoxification pathways.
Purpose of the Study:
- To investigate the potential protective role of manganese against the toxic effects of aflatoxin B1.
- To examine the biointeraction between manganese and aflatoxin B1 in vivo.
Main Methods:
- Young male Fischer rats were administered varying doses of aflatoxin B1 (AFB1) via intraperitoneal injection.
- Rats received radiolabeled manganese ([54Mn]-MnCl2) via gavage 12 hours post-AFB1 injection.
- Total body radioactivity and body weight changes were monitored over 72 hours post-radiolabel administration.
Main Results:
- Aflatoxin B1 exposure significantly altered the excretion and retention of manganese in rats.
- Higher doses of AFB1 led to increased initial manganese excretion followed by enhanced conservation of the metal at later time points.
- Aflatoxicosis was associated with reduced body weight gain in the treated animals.
Conclusions:
- The study provides evidence for a significant biointeraction between manganese and aflatoxin B1.
- Manganese metabolism is affected by AFB1 exposure, suggesting a potential role in aflatoxin toxicity or detoxification.