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Updated: Aug 17, 2026
Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
A dynamic, ultra-sensitive and "turn-on" strategy for fluorescent detection of uranyl based on DNAzyme and
Wen Yun1, Hong Wu2, Zhehan Yang2
1Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, Chongqing Technology and Business University, Chongqing, 400067, China; State Key Laboratory of Environment-Friendly Energy Material, Southwest University of Science and Technology, Mianyang 621010, PR China.
Abstract:
A uranyl detection strategy with ultra-sensitivity was developed based on entropy-driven amplification and DNAzyme circular cleavage amplification. The cleavage of UO22+-specific DNAzyme produces a DNA fragment to initiate the entropy-driven amplification. Two DNA sequences released from the entropy-driven amplification are partly complementary. They can form an entire enzyme strand (E-DNA) of Mg2+-specific DNAzyme. The formed E-DNA can circularly cleave FAM-labeled probes on gold nanoparticles (AuNPs), causing the leaving of FAM from AuNPs and recovery of fluorescent signal. A linear relationship was obtained in the range from 30 pM to 5 nM between fluorescence intensity and concentration of UO22+. The limit of detection was low to 13 pM. This method showed a promising future for practical application in real water samples.
