Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lattice Centering and Coordination Number02:33

Lattice Centering and Coordination Number

11.4K
The structure of a crystalline solid, whether a metal or not, is best described by considering its simplest repeating unit, which is referred to as its unit cell. The unit cell consists of lattice points that represent the locations of atoms or ions. The entire structure then consists of this unit cell repeating in three dimensions. The three different types of unit cells present in the cubic lattice are illustrated in Figure 1.
Types of Unit Cells
Imagine taking a large number of identical...
11.4K
Center of Gravity00:58

Center of Gravity

6.6K
The center of gravity (COG) of an object is the point where the object's total weight is considered to be concentrated. Knowing the location of the center of gravity is useful when predicting the behavior of a moving object or designing static structures. In a uniform gravitational field, the center of gravity is similar to the center of mass (COM); yet, these two points can be positioned differently. For example, the Moon's center of mass lies very close to its geometric center, but...
6.6K
Center of Gravity01:15

Center of Gravity

2.1K
The center of gravity is the point at which an object's weight appears to be concentrated and can be used to balance the object perfectly. This point is essential in mechanics as it provides information regarding a body's stability and moments of inertia. The center of gravity does not always have to fall within the shape or boundaries of the body; it may also lie outside the body in certain cases.
To determine its location, the principle of moments can be utilized by dividing the object into...
2.1K
Center of Mass00:59

Center of Mass

2.0K
The center of mass is the point at which the total mass of an object can be said to be concentrated. It is a fundamental principle in mechanics and physics that applies to all objects regardless of their shape or size. The center of gravity is the point at which an object’s weight appears to be concentrated and can be used to balance the object perfectly.
The knowledge of the center of mass can also help us to describe and predict the motion of objects. For example, when a ball is thrown...
2.0K
Instantaneous Center of Zero Velocity01:20

Instantaneous Center of Zero Velocity

819
General plane motion, often observed in a rolling wheel, refers to a type of movement where the wheel is simultaneously rotating and translating. This complex motion can be understood by breaking it down into individual components.
To analyze this, consider two points on the wheel: point A and point B. The absolute velocity of point B can be expressed as the vector sum of the absolute velocity of point A and the relative velocity of point B with respect to point A. To simplify this analysis,...
819
Patient-centered Care01:13

Patient-centered Care

2.9K
Patient-centered care involves delivering care beyond inpatient hospitalization. Reflective practice can enhance a patient-centered approach. Reflective practice is a process of reasoning that considers all aspects of the present situation, including practicalities, learning from personal practice, and consideration of patient needs. Patients appreciate care decisions made while considering their input. Involving the patient in their care provides the patient with a sense of contribution rather...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differentiating 5-thiooxazoles from oxazolone-coupled thioamides in RiPP natural products.

bioRxiv : the preprint server for biology·2026
Same author

A redox- and proton-coupled inner membrane transporter mediates copper import to the bacterial cytoplasm.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Bis-hydroxylation of Homocitrulline Catalyzed by a Multinuclear Nonheme Iron-Dependent Oxidative Enzyme during RiPP Biosynthesis.

bioRxiv : the preprint server for biology·2026
Same author

Sulfite Is Not Required for N<sub>2</sub> Reduction Catalyzed by Mo-Nitrogenase.

Journal of the American Chemical Society·2026
Same author

The radical SAM enzyme EpeE exhibits distinct site reactivity during the biosynthesis of the RiPP natural product epipeptide.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Simultaneous occupancy of Cu<sub>C</sub> and Cu<sub>D</sub> in the ammonia monooxygenase active site.

Chemical science·2026

Related Experiment Video

Updated: Jan 25, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
07:26

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Published on: January 31, 2025

818

Particulate methane monooxygenase contains only mononuclear copper centers.

Matthew O Ross1,2, Fraser MacMillan3, Jingzhou Wang4,5

  • 1Department of Molecular Biosciences, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA.

Science (New York, N.Y.)
|May 11, 2019
PubMed
Summary

Methane-oxidizing bacteria are key to reducing methane emissions. This study reveals two copper sites in particulate methane monooxygenase (pMMO), suggesting a single copper site can catalyze methane oxidation.

More Related Videos

Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders
06:52

Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders

Published on: April 28, 2023

1.9K
Methane Hydrate Crystallization on Sessile Water Droplets
08:46

Methane Hydrate Crystallization on Sessile Water Droplets

Published on: May 26, 2021

2.8K

Related Experiment Videos

Last Updated: Jan 25, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
07:26

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Published on: January 31, 2025

818
Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders
06:52

Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders

Published on: April 28, 2023

1.9K
Methane Hydrate Crystallization on Sessile Water Droplets
08:46

Methane Hydrate Crystallization on Sessile Water Droplets

Published on: May 26, 2021

2.8K

Area of Science:

  • Biochemistry
  • Environmental Microbiology
  • Biophysical Chemistry

Background:

  • Methane is a potent greenhouse gas, and bacteria that oxidize methane play a crucial role in its mitigation.
  • The active site of particulate methane monooxygenase (pMMO), the enzyme responsible for methane oxidation, has been a subject of debate due to conflicting research findings.
  • Understanding pMMO's structure and function is vital for developing strategies to control methane emissions.

Purpose of the Study:

  • To investigate the controversial nature of the copper active site in particulate methane monooxygenase (pMMO).
  • To characterize the biochemical and spectroscopic properties of pMMO to resolve discrepancies in previous studies.
  • To propose a model for the copper active site(s) involved in methane oxidation by pMMO.

Main Methods:

  • Biochemical characterization of particulate methane monooxygenase (pMMO).
  • Electron paramagnetic resonance (EPR) spectroscopy to probe copper sites.
  • Analysis of spectroscopic and biochemical data to determine the number and location of copper centers.

Main Results:

  • Evidence for two distinct monocopper sites within pMMO was found.
  • One copper site (CuB) is located in the soluble PmoB subunit.
  • A second copper site (CuC) is situated in the membrane-bound PmoC subunit, approximately 2 nm from CuB.

Conclusions:

  • The findings suggest that pMMO contains two monocopper sites, challenging previous models.
  • A single monocopper site is proposed to be catalytically active in methane oxidation.
  • This research provides a new perspective on the mechanism of methane oxidation by pMMO.