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MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
Marie-Alice Fraiture1, Gabriella Ujhelyi2, Jaroslava Ovesná3
1Sciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
Abstract:
In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.
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