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Generation and Selection of a Panel of Pan-Filovirus Single-Chain Antibodies using Cell-Free Ribosome Display

Adinarayana Kunamneni1,2, Elizabeth C Clarke2, Chunyan Ye2

  • 1Department of Medicine, Loyola University Medical Center, Chicago, Illinois.

The American Journal of Tropical Medicine and Hygiene
|May 11, 2019
PubMed

Insights

Researchers developed new mouse antibodies (scFvs) to detect all known ebolaviruses and marburgviruses. These rapid filovirus diagnostics offer potential for public health and biodefense applications.

Area of Science:

  • Virology
  • Immunology
  • Biotechnology

Background:

  • Filoviruses, including ebolaviruses and marburgviruses, cause lethal hemorrhagic fevers with high mortality rates.
  • Current treatments for filovirus infections are limited, and early symptoms resemble common diseases, necessitating rapid diagnostics.

Purpose of the Study:

  • To develop broad-spectrum diagnostic tools for filoviruses.
  • To generate and characterize novel antibodies targeting filovirus glycoproteins (GPs).

Main Methods:

  • Utilized cell-free ribosome display to generate a panel of mouse single-chain Fv-antibodies (scFvs).
  • Assessed the cross-reactivity of generated scFvs against all known filovirus species.

Main Results:

  • Identified two scFvs (4-2 and 22-1) capable of detecting all tested ebolavirus and marburgvirus species.
  • This is the first report of ribosome display-derived scFvs with broad reactivity to filovirus GPs.

Conclusions:

  • The developed scFvs show significant potential for use in rapid, broad-spectrum filovirus diagnostic assays.
  • These findings address a critical unmet need in public health and biodefense for filovirus detection.

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