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High-Precision Quantitation of Biofluid Samples Using Direct Mass Spectrometry Analysis
Wenpeng Zhang1,2, Yue Ren3, Ziqing Lin3
1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument , Tsinghua University , Beijing 100084 , China.
Analytical Chemistry
|May 11, 2019
Summary
A new method simplifies direct quantitation in biofluid samples using mass spectrometry. This technique enhances clinical analysis by enabling rapid and accurate drug compound measurements in urine and blood.
Area of Science:
- Analytical Chemistry
- Clinical Diagnostics
- Mass Spectrometry
Background:
- Direct sampling ionization simplifies mass spectrometry for clinical analysis.
- High-precision quantitation remains a significant challenge in the clinical application of mass spectrometry.
- Existing methods for incorporating internal standards are complex and limit operational simplicity.
Purpose of the Study:
- To develop a novel, simplified method for the direct quantitation of biofluid samples using mass spectrometry.
- To overcome the challenges associated with high-precision quantitation in clinical settings.
- To establish a protocol with a significant impact on on-site or clinical analysis.
Main Methods:
- Developed a novel method for direct quantitation of biofluid samples.
- Utilized slug flow microextraction for method development.
- Employed internal standards with partition coefficients significantly different from analytes and large sample-to-extraction solvent volume ratios, guided by a theoretical model.
Main Results:
- Demonstrated direct quantitation of drug compounds in both urine and blood samples.
- Achieved high-precision quantitation with a significantly simplified procedure.
- Validated the theoretical model's predictions for internal standard selection and volume ratios.
Conclusions:
- The developed method offers an extremely simplified protocol for direct biofluid quantitation.
- This advancement is expected to significantly impact on-site and clinical mass spectrometry analysis.
- The novel approach addresses the critical need for high-precision quantitation in clinical settings.
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