Related Experiment Video
Updated: Jan 25, 2026

Guided Endodontics: Three-Dimensional Planning and Template-Aided Preparation of Endodontic Access Cavities
Published on: May 24, 2022
In Vitro Effect of Er:YAG Laser on Different Single and Mixed Microorganisms Being Associated with Endodontic
Eva Henninger1, Luciana Aranha Berto1, Sigrun Eick2
11 Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland.
Abstract:
The purpose of this in vitro study was to evaluate the antimicrobial effect of activated irrigation with different modes of erbium-doped yttrium aluminum garnet (Er:YAG) laser application on microorganisms related to secondary endodontic infection. Er:YAG laser has been recommended as an adjuvant tool for root canal disinfection during endodontic treatment. Laser-activated irrigation (LAI) with 300 or 600 μm tips were tested with or without intermittent irrigation with 0.9% sodium chloride (NaCl) solution against different microorganisms (five single strains and dual species (Streptococcus gordonii combined with Actinomyces oris or Fusobacterium nucleatum) in root canals after 3 days of incubation. In a 21-day infection model, LAI was used together with intermittent rinsing with sodium hypochlorite (NaOCl) against the dual-species mixtures; here the incidence of microbial regrowth after up to 7 days was monitored. In the 3-day root infection model, LAI protocols did not show any significant reduction of the microbial load when compared with manual irrigation with saline solution. In the 21-day infection, S. gordonii combined with A. oris were not detectable anymore after applying the LAI protocol with a 600 μm tip (30 mJ/10 pps) up to 7 days after treatment. Application of LAI with a 600 μm tip by using an Er:YAG laser might be advantageous in treatment of endodontic infections.
More Related Videos
Related Concept Videos
Types of Microorganisms
Mutations in Microorganisms
Environmental Applications of Microorganisms
Microorganisms in Medicine and Therapeutics
Microorganisms in Agriculture and Food industry
Mixing Concrete

