Related Experiment Video
Updated: Jan 25, 2026
11:54
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
Published on: May 26, 2009
20.0K
Strategy towards tailored donor tissue-specific pancreatic islet isolation
Yuki Miyazaki1, Kazutaka Murayama2, Ibrahim Fathi3
1Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
Plos One
|May 11, 2019
Summary
Optimizing the collagenase G (ColG) to collagenase H (ColH) ratio is crucial for islet isolation. Pancreatic collagen III (Col III) expression and collagen I (Col I) composition, determined by mass spectrometry, aid in tailoring this ratio for improved outcomes.
Area of Science:
- Biochemistry
- Cell Biology
- Endocrinology
Background:
- Optimizing islet isolation relies on precise collagenase ratios, specifically collagenase G (ColG) and collagenase H (ColH).
- Collagen V (Col V) and collagen III (Col III) are key targets for ColG and ColH, respectively.
- Understanding the relationship between pancreatic matrix composition and enzyme ratios is vital for successful islet isolation.
Purpose of the Study:
- To investigate the correlation between pancreatic target matrix expression and islet isolation efficiency.
- To determine the optimal collagenase G (ColG):collagenase H (ColH) ratio for islet isolation based on tissue-specific collagen composition.
Main Methods:
- Islet isolation was performed in Lewis and Sprague-Dawley (SD) rats using varying ColG:ColH ratios (5:1, 1:1, 1:5).
- Collagen III (Col III) and Collagen V (Col V) expression was analyzed via immunohistochemistry, real-time PCR, Western blotting, and mass spectrometry.
- Collagen I (Col I) chain composition was assessed using mass spectrometry.
Main Results:
- Increasing ColG did not improve islet yield in either rat strain.
- High ColH enhanced islet yield in Lewis rats but decreased it in SD rats, indicating differential Col III content.
- Western blotting showed significantly higher Col III in Lewis than SD rats, with lower Col-I(α1)/Col-III(α1) and Col-I(α2)/Col-III(α1) ratios.
- Islet isolation outcomes correlated significantly with the composition of homotrimeric Col I.
Conclusions:
- Pancreatic collagen III (Col III) expression levels are critical for determining the optimal ColG:ColH ratio.
- Mass spectrometry analysis of Col III and homotrimeric collagen I composition provides valuable insights for refining islet isolation protocols.
- Tailoring the ColG:ColH ratio based on tissue-specific collagen profiles can optimize islet isolation efficiency.
Related Concept Videos
01:27Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
2.2K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
Insulin and C-peptide are...
2.2K
01:52Persuasion Strategies
43.0K
Researchers have tested many persuasion strategies, including the foot-in-the door and the door-in-the-face techniques, in a variety of contexts. Ultimately, the principles are effective in selling products and changing people’s attitude, ideas, and behaviors (Cialdini & Goldstein, 2004).
43.0K
01:27Coping Strategies: Problem Focused
450
Coping strategies are methods people use to manage, tolerate, or reduce the effects of stressors. These strategies involve both behavioral and psychological actions to handle stressful situations. One common approach is problem-focused coping, which aims to change or eliminate the source of stress rather than merely addressing its consequences. This method involves taking direct action to resolve the issue causing stress.
For example, consider a student who struggles to understand their...
For example, consider a student who struggles to understand their...
450
01:26Pancreatic Juice and Secretion
2.9K
Pancreatic juice is a clear fluid produced by the pancreas, containing water, salts, sodium bicarbonate, and enzymes vital for digestion in the small intestine. It helps break down large molecules, facilitating nutrient absorption.
When acidic chyme from the stomach enters the duodenum, it triggers the release of secretin, a hormone that prompts pancreatic juice secretion. After a fatty meal, cholecystokinin, another hormone, stimulates gallbladder contraction and enhances enzyme-rich...
When acidic chyme from the stomach enters the duodenum, it triggers the release of secretin, a hormone that prompts pancreatic juice secretion. After a fatty meal, cholecystokinin, another hormone, stimulates gallbladder contraction and enhances enzyme-rich...
2.9K
01:27Acute Pancreatitis I: Introduction
1.2K
Pancreatitis is inflammation of the pancreas, an organ located behind the stomach. It can be either acute or chronic.
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
1.2K
01:24Chronic Pancreatitis I: Introduction
688
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
688