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Related Experiment Video

Updated: Jan 25, 2026

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
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Cdk5 regulates N-cadherin-dependent neuronal migration during cortical development.

Dong-Keun Lee1, Hojae Lee1, Jiyoung Yoon1

  • 1School of Life Sciences, Gwangju Institute of Science and Technology, Oryong-dong, Buk-gu, Gwangju, 500-712, Republic of Korea.

Biochemical and Biophysical Research Communications
|May 12, 2019
PubMed
Summary

Cyclin-dependent kinase 5 (Cdk5) regulates neuronal migration by modulating N-cadherin (Ncad) cell adhesion. This interaction is crucial for the multipolar to bipolar transition of developing neurons, ensuring proper cortical development.

Keywords:
Cdk5N-cadherinNeuronal locomotion

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neuronal migration is critical for forming the developing cerebral cortex.
  • Cyclin-dependent kinase 5 (Cdk5) is known to control neuronal migration.
  • The precise mechanisms by which Cdk5 influences cell adhesion during migration remain unclear.

Purpose of the Study:

  • To investigate the functional interaction between Cdk5 and N-cadherin (Ncad) in migrating neurons.
  • To elucidate the role of Cdk5 in regulating cell adhesion during the multipolar to bipolar transition in the intermediate zone (IZ).

Main Methods:

  • Expression analysis of Cdk5 and Ncad in migrating neurons.
  • In vivo misexpression of dominant-negative Cdk5 (Cdk5DN) and Ncad in embryonic brains.
  • In vitro studies involving downregulation of Cdk5 and subsequent Ncad expression.
  • Analysis of cell morphology, migration, and aggregation.
  • Assessment of Ncad surface expression.

Main Results:

  • Cdk5 and Ncad are co-expressed in migrating neurons within the IZ.
  • Cdk5 inhibition stalls neuronal migration and results in multipolar cell morphology.
  • Co-introduction of Ncad rescues migration and promotes bipolar morphology.
  • Cdk5 downregulation reduces cell aggregation, which is reversed by Ncad.
  • Cdk5 activity affects surface expression of Ncad without altering total Ncad levels.
  • Cdk5 and Ncad expression patterns are conserved in the human fetal cortex.

Conclusions:

  • Cdk5 directly interacts with Ncad to control cell adhesion during neuronal migration.
  • This Cdk5-Ncad interaction is essential for the multipolar to bipolar transition of cortical neurons.
  • The findings highlight a conserved mechanism regulating neuronal development across species.