FAT1 cadherin controls neuritogenesis during NTera2 cell differentiation
Abdulrzag F Ahmed1, Charles E de Bock2, Estelle Sontag3
1Department of Pharmacology, Faculty of Pharmacy, Elmergib University, Alkhoms, Libya; School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, 2308, Australia.
Biochemical and Biophysical Research Communications
|May 12, 2019
Summary
Fat1 cadherin is crucial for neuronal differentiation. Its depletion in the Ntera2 cell model reduced neurite formation and altered Hippo signaling, highlighting Fat1
Area of Science:
- Neuroscience
- Cell Biology
- Molecular Biology
Background:
- Fat1 cadherin is widely expressed in the nervous system.
- Fat1 has been linked to neuronal differentiation processes.
Purpose of the Study:
- To investigate the role of Fat1 in neuronal differentiation using the Ntera2 cell line model.
- To understand Fat1's contribution to neuritogenesis and associated signaling pathways.
Main Methods:
- Utilized the Ntera2 cell line for neuronal differentiation studies.
- Employed retinoic acid (RA) to induce differentiation.
- Used small interfering RNA (siRNA) to deplete FAT1 expression.
- Assessed neurite outgrowth and YAP phosphorylation.
- Monitored the expression of Hippo pathway target genes (CTGF, ANKRD1).
Main Results:
- FAT1 expression increased during RA-induced differentiation of NTera2 cells.
- Depletion of FAT1 using siRNA significantly reduced neurite production.
- FAT1 silencing led to decreased YAP phosphorylation at Ser127.
- Transcriptional upregulation of Hippo target genes CTGF and ANKRD1 was observed upon FAT1 depletion.
Conclusions:
- FAT1 plays a critical role in regulating neurite formation during early neuronal differentiation.
- FAT1 influences the Hippo signaling pathway during the differentiation of NTera2 cells.
- FAT1 is essential for efficient neuritogenesis in this cellular model.
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