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Updated: Jan 25, 2026

Simplified Human Neutrophil Extracellular Traps NETs Isolation and Handling
Published on: April 16, 2015
Use of hair nets and face masks to decrease blood culture contamination rates
Amanda M Sanders1, William A Agger2, Alicia M Gray3
1Department of Microbiology, Gundersen Health System, 1900 South Avenue, La Crosse, WI, 54601, USA.
Abstract:
Owing to a persistently high blood culture contamination rate of 3.2% exceeding the target rate of <3%, a midwestern United States hospital began a series of 3 additive interventions. After collecting phlebotomist data for approximately 3 months, reporting of individual contamination rates commenced. A specialized trainer reeducated staff with high rates, which resulted in a modest decrease in contamination rates (3.2% to 2.8%, P = 0.23). A second, additional intervention requiring phlebotomists to wear hair nets and face masks resulted in marked improvement from a mean of 2.8% to 1.1% (P < .0001). In a final, third addition, whenever possible, the replacement of nursing staff by phlebotomy staff for blood specimen collection did not result in a significant change in mean contamination (P = 0.81). Overall, the mean contamination rate progressively declined in a stepwise manner from 3.2% to 1.2% (P = .0013), with the greatest decline after adding hair nets and face masks.
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