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FEZF1-AS1 functions as an oncogenic lncRNA in retinoblastoma

Lian-Jiao Quan1, Wen-Jun Wang2,3,4

  • 1Department of Ophthalmology, Qingyang People's Hospital, Qingyan, Gansu 745000, China.

Bioscience Reports
|May 12, 2019
PubMed

Insights

Long non-coding RNA FEZF1 antisense RNA 1 (FEZF1-AS1) is elevated in retinoblastoma, correlating with advanced disease and poor survival. Silencing FEZF1-AS1 inhibits tumor cell growth and spread, identifying it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Ophthalmology

Background:

  • Long non-coding RNA (lncRNA) FEZF1 antisense RNA 1 (FEZF1-AS1) is implicated as an oncogene in various cancers.
  • The role of FEZF1-AS1 in retinoblastoma, a rare eye cancer, remains largely uncharacterized.

Purpose of the Study:

  • To investigate the expression patterns of FEZF1-AS1 in retinoblastoma.
  • To evaluate the clinical significance of FEZF1-AS1 expression in retinoblastoma patients.
  • To explore the functional role of FEZF1-AS1 in retinoblastoma cell behavior.

Main Methods:

  • Quantitative real-time PCR was used to measure FEZF1-AS1 expression levels in tumor and normal tissues/cell lines.
  • Correlation analyses were performed to associate FEZF1-AS1 expression with clinical parameters.
  • Survival analysis (Kaplan-Meier) was conducted to assess the prognostic value of FEZF1-AS1.
  • In vitro loss-of-function experiments (siRNA-mediated silencing) were employed to study FEZF1-AS1's effects on cell proliferation, migration, and invasion.

Main Results:

  • FEZF1-AS1 expression was significantly upregulated in retinoblastoma tissues and cell lines compared to controls.
  • High FEZF1-AS1 expression correlated significantly with choroidal and optic nerve invasion.
  • Elevated FEZF1-AS1 levels were associated with shorter disease-free survival (DFS) and identified as an independent unfavorable prognostic factor.
  • Silencing FEZF1-AS1 suppressed retinoblastoma cell proliferation, migration, and invasion.

Conclusions:

  • FEZF1-AS1 is overexpressed in retinoblastoma and serves as a potential biomarker for disease progression and prognosis.
  • FEZF1-AS1 promotes retinoblastoma cell proliferation, migration, and invasion, acting as an oncogenic lncRNA.
  • Targeting FEZF1-AS1 may offer a novel therapeutic strategy for retinoblastoma.
Keywords:
FEZF1-AS1biomarkerscancerlarge intervening non-coding RNAretinoblastoma

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