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Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
14:05

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Published on: September 19, 2013

10.3K

Listeria monocytogenes hijacks CD147 to ensure proper membrane protrusion formation and efficient bacterial

Aaron S Dhanda1, Katarina T Lulic1, Connie Yu1

  • 1Department of Biological Sciences, Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, BC, Canada.

Cellular and Molecular Life Sciences : CMLS
|May 12, 2019
PubMed

Insights

Listeria monocytogenes uses the host protein CD147 to form membrane protrusions for cell-to-cell spread. Depleting CD147 impairs bacterial dissemination by affecting protrusion formation.

Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Efficient cell-to-cell transfer of Listeria monocytogenes relies on actin-rich membrane protrusions.
  • Host proteins ezrin, CD44, and cyclophilin A (CypA) are known to stabilize these protrusions.

Purpose of the Study:

  • To investigate the role of CD147, a binding partner of CypA, in Listeria monocytogenes cell-to-cell dissemination.
  • To determine if CD147 is involved in the formation and stabilization of bacterial membrane protrusions.

Main Methods:

  • Examined CD147 localization in host cells during Listeria monocytogenes infection.
  • Assessed the impact of CD147 depletion on actin-rich membrane protrusion formation.
  • Evaluated the efficiency of Listeria monocytogenes cell-to-cell transfer in CD147-depleted cells.

Main Results:

  • CD147 is enriched at membrane protrusions and invaginations during Listeria monocytogenes infection.
  • Depletion of CD147 leads to shorter, more contorted membrane protrusions, similar to CypA-depleted cells.
  • Malformed protrusions in CD147-depleted cells significantly hinder Listeria monocytogenes dissemination.

Conclusions:

  • CD147 is a novel host protein hijacked by Listeria monocytogenes for cellular dissemination.
  • CD147 plays a crucial role in the formation and stabilization of membrane protrusions required for bacterial spread.
  • Targeting CD147 could represent a strategy to inhibit Listeria monocytogenes intercellular transfer.
Keywords:
ActinActin-based motilityCell-to-cell spreadingComet/rocket tailsInfectious diseaseMicrobiology

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