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Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry

Diana Shinko1, Thomas M Ashhurst2,3,4,5, Helen M McGuire6,7,8

  • 1Discipline of Pharmacology, School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|May 12, 2019
PubMed
Summary

This study optimizes mass cytometry protocols for analyzing human blood samples. The enhanced method allows for detailed analysis of intracellular signaling pathways, including phosphorylated proteins, at a single-cell level.

Keywords:
Inflammatory pathwaysMass cytometryPhosphorylationSignaling pathwaySignaling proteins

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