What is the fate of xylem-transported CO₂ in Kranz-type C₄ plants?

High concentrations of dissolved inorganic carbon in stems of herbaceous and woody C₃ plants exit leaves in the dark. In the light, C₃ species use a small portion of xylem-transported CO₂ for leaf photosynthesis. However, it is not known if xylem-transported CO₂ will exit leaves in the dark or be used for photosynthesis in the light in Kranz-type C₄ plants. Cut leaves of Amaranthus hypochondriacus were placed in one of three solutions of [NaH¹³ CO₃ ] dissolved in KCl water to measure the efflux of xylem-transported CO₂ exiting the leaf in the dark or rates of assimilation of xylem-transported CO₂ * in the light, in real-time, using a tunable diode laser absorption spectroscope. In the dark, the efflux of xylem-transported CO₂ increased with increasing rates of transpiration and [¹³ CO₂ *]; however, rates of ¹³ C_efflux in A. hypochondriacus were lower compared to C₃ species. In the light, A. hypochondriacus fixed nearly 75% of the xylem-transported CO₂ supplied to the leaf. Kranz anatomy and biochemistry likely influence the efflux of xylem-transported CO₂ out of cut leaves of A. hypochondriacus in the dark, as well as the use of xylem-transported CO₂ * for photosynthesis in the light. Thus increasing the carbon use efficiency of Kranz-type C₄ species over C₃ species.