Immunohistochemical localization of guanine nucleotide-binding protein in rat retina

The localization of a guanine nucleotide-binding protein, Go, in rat retina has been immunohistochemically examined by use of affinity-purified antibody against the alpha-subunit of Go. Dense Go-immunoreactive products are localized in the inner and outer plexiform layers which are considered as the region of synaptic interplay between neuronal components of the retina. Weak Go-immunoreactivity is also found in the neuropil of the ganglion cell layer and the inner nuclear layer. The selective enrichment of Go in synaptic zones of the retina suggests its major role in neuronal transmission within the retina.